By N. Purdie, Harry G. Brittain
Gains specific features of round dichroism and examines the way it may be of information to employees within the box. The textual content discusses the actual phenomena linked to dissymmetric compounds which provide upward thrust to the round dichroism impression, and experiences the instrumentation on hand.
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Extra info for Analytical Applications of Circular Dichroism
In conventional approaches, the monochromator is placed after the source in order to limit the UV exposure of the sample. However, in the multichannel arrangement, the polychromator is located after the sample to allow the sample to be 29 exposed to all wavelengths simultaneously. The CCD can be configured such that a group of elements will define a specific wavelength interval, and exposure data for these elements allows the sample absorption at that wavelength to be determined. In one complete cycle, the CCD will be readout twice, thereby providing sample transmittances at the specified wavelength for both left- and right-circularly polarized light.
The light source utilized for the stopped-flow CD system was a high pressure mercury arc, as it can provide a high spectral radiance over the spectral range 280 - 600 nm. The output of this source contains many lines from atomic mercury superimposed on a xenon continuum background, and the radiance can exceed that of the Xe arc by a factor of 1020 over its usable range. The limitation to this source is its instability, as intensity fluctuations are usually large and significant. This problem was solved by splitting off a portion of the source light before the polarizing optics with a beamsplitter.
For multichannel detection, the monochromator has been replaced by a polychromator which does not possess an exit slit to define a wavelength interval. For multichannel detection, the location (pixel) on the multichannel device defines the wavelength of interest. In conventional approaches, the monochromator is placed after the source in order to limit the UV exposure of the sample. However, in the multichannel arrangement, the polychromator is located after the sample to allow the sample to be 29 exposed to all wavelengths simultaneously.
Analytical Applications of Circular Dichroism by N. Purdie, Harry G. Brittain